7/12/2023 0 Comments Sf2 3d structure![]() ![]() Mutagenesis of Hck SH3 Glu-93 interfered with Nef A new intercomplex contact was found between SH3 Glu-93, and Nef Arg-105. The complex crystallized as a dimer of complexes, with the conserved Nef PXXPXR motif engaging the Hck SH3 domain. Here we describe the x-ray crystal structure of Nef in complex with the Hck SH3-SH2 regulatory region to a resolution of 1.86 Å. Nef function depends on interactions with multiple host cell effectors, including Hck and other Src-family kinases. HIV-1 Nef supports high titer viral replication in vivo and is essential for AIDS progression.
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